Thermo Fisher Scientific PGR Monoclonal Antibody (1B11)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

ELISA

• Tested Dilution: 10 µg/mL

Product Specifications

Product Specific Information

Sequence of this protein is as follows:
MTELKAKGPR APHVAGGPPS PEVGSPLLCR PAAGPFPGSQ TSDTLPEVSA IPISLDGLLF PRPCQGQDPS DEKTQDQQSL SDVEGAYSRA EATRGAGGSS SSPPEKDSGL

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy.

PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form responsible for activating genes for endometrium maintenance, pregnancy, and inhibition of ovulation.

PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus, exposing a 140 amino acid inhibitory domain (ID) that represses steroid hormone transcriptional activity.

In its inactive state, PgR forms a multiprotein complex including heat shock proteins and immunophilins. Upon binding of progesterone, conformational change enables dimerization and binding to progesterone response elements (PRE), activating transcription.

A null mutation in the PGR gene leads to pleiotropic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.