Thermo Fisher Scientific REST/NRSF Polyclonal Antibody, FITC
REST/NRSF 단백질을 인식하는 FITC 표지 폴리클로날 항체로, Western blot, ELISA, IP에 적합합니다. 사람, 생쥐, 랫드 시료에 반응하며, 친화 크로마토그래피로 정제되었습니다. 액상 형태로 -20°C 암소 보관이 권장됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 1000–1097 on human REST protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
REST (RE1-Silencing Transcription factor, also known as NRSF) is a transcriptional repressor that binds to neuron-restrictive silencer elements (NRSE) and represses neuronal gene transcription in non-neuronal cells. It restricts neuronal gene expression by associating with corepressors such as mSin3 and CoREST, which recruit histone deacetylases to REST-regulated gene promoters. REST mediates repression by recruiting the BHC complex at RE1/NRSE sites, leading to histone deacetylation and demethylation, thereby modifying chromatin structure.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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