Thermo Fisher Scientific ZNF322A Polyclonal Antibody
Human ZNF322A 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(P)에서 검증됨. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 연구용으로 MAPK 신호전달 관련 전사 조절 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ZNF322A. Recombinant protein control fragment (Product # RP-99502) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2650067 |
Product Specific Information
Immunogen sequence:
QRIHMEEKPH QWSACESGFL LGMDFVAQQK MRTQTEELHY KYTVCD
Antigen sequence identity:
Mouse – 91%, Rat – 91%
Target Information
ZNF322 contains four exons and spans 23.2 kb in the chromosome 6p22.1 region, and transcribes a 2.7 kb mRNA encoding a protein with 402 amino acid residues.
Northern blot analysis shows ZNF322 expression in all human tissues examined at adult and embryonic stages (80 days to 24 weeks).
When overexpressed in COS-7 cells, ZNF322–EGFP fusion protein localizes to both nucleus and cytoplasm.
Reporter gene assays indicate ZNF322 functions as a transcriptional activator.
Overexpression of ZNF322 activates transcriptional activity of SRE and AP-1, suggesting that ZNF322 is a zinc-finger transcription factor involved in positive regulation of gene transcription mediated by MAPK signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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