Thermo Fisher Scientific Human FGF-basic (FGF-2/bFGF) (154 aa), Animal-Free Recombinant Protein, PeproTech
Animal-free recombinant human FGF-basic (FGF-2/bFGF) 단백질로, 줄기세포 연구에 적합하며 고순도(≥95%)와 낮은 엔도톡신(<0.1 EU/µg)을 특징으로 함. 세포 증식 활성 확인됨(ED50 ≤ 0.1 ng/ml). 상온 배송, -20°C 보관.
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Applications
Western Blot (WB)
- Tested Dilution: -
- View 1 publication
Functional Assay
- Assay-dependent
- View publication
In vitro Assay (IV)
- Tested Dilution: -
- View 224 publications
Miscellaneous PubMed (Misc)
- Tested Dilution: -
- View 30 publications
Immunostaining (IS)
- Tested Dilution: -
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published species | Amphibian, Bovine, Chicken, Dog, Hamster, Horse, Human, Mouse, Non-human primate, Rabbit, Rat, Virus, Zebrafish |
| Expression System | E. coli |
| Amino acid sequence | AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS |
| Molecular weight | 17.2 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 95% by SDS-PAGE gel and HPLC analyses |
| Endotoxin concentration | <0.1 EU/µg |
| Activity | Determined by a cell proliferation assay using Balb/c 3T3 cells. Expected ED50 ≤ 0.1 ng/ml, corresponding to specific activity ≥ 1 × 10⁷ units/mg |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
Product Specific Information
Recombinant Human FGF-basic is a 17.2 kDa protein consisting of 154 amino acid residues. Qualified for stem cell use.
This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis.
Target Information
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization.
FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24, and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways.
In humans, the gene is located on the q arm of chromosome 4. FGF2 is involved in limb and nervous system development, wound healing, and tumor growth.
The mRNA for FGF2 contains multiple polyadenylation sites and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms.
The CUG-initiated isoforms are nuclear and responsible for intracrine effects, while the AUG-initiated form is cytosolic and responsible for paracrine and autocrine effects.
Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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