Thermo Fisher Scientific PIM2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 53 and 278 of PIM2 (Uniprot ID#Q9P1W9) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PIM2,uniProtId:Q9P1W9-1,ncbiNodeId:9606,antigenRange:53-278,antigenLength:311,antigenImageFileName:PA5-22315_PIM2_Q9P1W9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-22315_PIM2_Q9P1W9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11152131

Product Specific Information

Recommended positive controls: 293T, mouse testis.

Predicted reactivity: Mouse (91%), Rat (91%), Rhesus Monkey (99%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PIM2 is a proto-oncogene which phosphorylates BAD, which partially inhibits its apopotopic function. PIM proteins (PIM-1, PIM-2 and PIM-3) are oncogene-encoded serine/threonine kinases. PIM-2 is highly homologous to Pim-1 with similar oncogenic functions. PIM-2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. PIM2 expression is negatively regulated by growth factor depletion. Increased levels of PIM-2 has also been observed in certain cancers.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.