Thermo Fisher Scientific Pc2 Polyclonal Antibody
Thermo Fisher Scientific의 Pc2 Polyclonal Antibody는 인간 및 마우스 반응성을 가진 Rabbit IgG 폴리클로날 항체로, WB, IHC, IP에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 4°C에서 보관. 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 150 and 200 of human polycomb 2 (chromobox homolog 4) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
Polycomb group (PcG) proteins are chromatin-associated proteins important for embryonic and adult stem cell self-renewal and maintenance. At least two distinct human PcG complexes have been identified. Polycomb repressive complex I (PRC1) contains CBX4/Pc2, HPH1, and RING domain-containing proteins (RING1, RING2, and BMI1). CBX4 (Chromobox protein homolog 4 or PC2) functions as a transcriptional suppressor and acts as an E3 SUMO-protein ligase facilitating SUMO1 conjugation. Depletion of CBX4 decreases cellular resistance to ionizing radiation and is directly involved in the cellular response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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