Thermo Fisher Scientific Human Osteoactivin (GPNMB) ProcartaPlex Simplex Kit

Invitrogen™ Human Osteoactivin (GPNMB) ProcartaPlex™ Simplex Kit

The Human Osteoactivin (GPNMB) ProcartaPlex Simplex Kit measures osteoactivin (GPNMB) protein and is designed to be combinable with other Simplex kits to create custom multiplex panels utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits, only one buffer kit (sold separately) is needed per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the sandwich ELISA principle, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument.
These multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and deliver analyzed results within four hours.

Features

• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX).
Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are detected using biotinylated antibodies and streptavidin–R-phycoerythrin (RPE).

Unlike traditional ELISA, the capture antibody in ProcartaPlex assays is conjugated to a bead rather than adsorbed to a microplate well, allowing reagents to remain free-floating. The Luminex instrument uses two lasers — one to identify bead signatures and another to quantify RPE fluorescence, proportional to protein amount. This enables profiling of more targets with less sample in comparable assay time.

ProcartaPlex multiplex panels are available across six species (human, mouse, rat, nonhuman primate, porcine, canine).
For more information, visit thermofisher.com/procartaplex.

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