Thermo Fisher Scientific IRS1 Monoclonal Antibody (OTI1D10), TrueMAB

Thermo Fisher Scientific IRS1 Monoclonal Antibody (OTI1D10), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution: 1:2,000

Species Reactivity

Human, Mouse, Rat

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

IRS-1 is a major substrate of the insulin receptor and is phosphorylated in response to stimulation by insulin, IGF-1, and IL-4. It undergoes phosphorylation on serine, threonine, and tyrosine residues in various tissues. Casein kinase II mediates a portion of insulin-stimulated serine/threonine phosphorylation of IRS-1 in vivo, with Thr502 and Ser99 identified as major phosphorylation sites.
IRS-1 also contains three putative 14-3-3 binding sites (Ser270, Ser374, Ser641), with the Ser270 motif located in the phosphotyrosine binding domain responsible for interaction with the insulin receptor.

Usage Note

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.