Thermo Fisher Scientific IRS1 Monoclonal Antibody (OTI2B10), TrueMAB
IRS1 단백질을 인식하는 마우스 단일클론 항체로 Western blot 및 IHC(P) 실험에 적합. 인간, 마우스, 랫트 반응성. 무보존제, 동결건조 형태로 제공되며 재구성 및 접합 실험에 용이. 연구용으로만 사용 가능.
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Thermo Fisher Scientific IRS1 Monoclonal Antibody (OTI2B10), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–280 of human IRS1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform desalting (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
IRS-1 is a major substrate of the insulin receptor and is phosphorylated in response to stimulation by insulin, IGF-1, and IL-4. It undergoes phosphorylation on serine, threonine, and tyrosine residues in various tissues. Casein kinase II mediates insulin-stimulated serine/threonine phosphorylation of IRS-1, with Thr502 and Ser99 identified as major sites. IRS-1 also contains three potential 14-3-3 binding sites (Ser270, Ser374, Ser641), with Ser270 located in the phosphotyrosine binding domain responsible for interaction with the insulin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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