Thermo Fisher Scientific PDCD4 Polyclonal Antibody

Applications

• Western Blot (WB): 0.2–2.5 µg/mL

Product Specifications

Product Specific Information

• Recommended positive controls: HepG2, Jurkat
• Predicted reactivity: Cow 100%, Dog 100%, Goat 100%, Guinea Pig 100%, Mouse 100%, Rabbit 100%, Rat 100%
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. During this process, the expression patterns of numerous genes are radically altered. One such gene is the programmed cell death protein 4 (PDCD4), whose expression is upregulated following the onset of apoptosis. PDCD4 encodes a tumor suppressor protein whose expression is lost in carcinomas of breast, colon, lung, and prostate. It can bind to and inhibit the helicase activity of the eukaryotic translation initiation factor 4A and inhibit the transactivation and transformation mediated by the transcription factor AP-1. The kinase Akt regulates PDCD4 by phosphorylation, decreasing the ability of PDCD4 to interfere with the transactivation of AP-1-responsive promoter by c-Jun. There are two known isoforms of PDCD4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.