Thermo Fisher Scientific EMMPRIN Human ProcartaPlex Simplex Kit

Invitrogen™ EMMPRIN Human ProcartaPlex™ Simplex Kit

The Human EMMPRIN Simplex ProcartaPlex Kit measures EMMPRIN protein and is designed to be combinable with other Simplex kits, allowing you to create your own multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation. When combining multiple Simplex kits (i.e., when not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is required per assay plate, regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and deliver analyzed results within four hours.

• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.

The Luminex beads used in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures identifiable by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to distinct beads allows analysis of multiple targets in a single well.

The key difference between a ProcartaPlex assay and a traditional ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead rather than adsorbed to a microplate well, allowing reagents to remain free-floating in solution. Detection is achieved using a Luminex instrument equipped with two lasers — one distinguishing the bead’s spectral signature and another quantifying RPE fluorescence proportional to protein concentration. ProcartaPlex multiplex assays enable profiling of more targets using significantly less sample and time compared to conventional ELISA.

ProcartaPlex multiplex panels are available across six species: human, mouse, rat, nonhuman primate, porcine, and canine.

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