Thermo Fisher Scientific Fibronectin Recombinant Rabbit Monoclonal Antibody (23GB1335)
인간 Fibronectin 단백질을 인식하는 토끼 유래 재조합 단일클론 항체. Western blot과 면역세포화(ICC/IF)에 적합. 고순도 친화 크로마토그래피 정제, PBS/glycerol 완충액에 보관. 세포 부착 및 이동 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293F |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 23GB1335 |
| Immunogen | A synthesized peptide derived from human Fibronectin (1–2477 AA) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice (domestic); Dry ice (international) |
Target Information
Fibronectin is a disulfide-bonded dimer with a molecular weight of 230–250 kDa. In the extracellular matrix of connective tissues and vessels, fibronectin exists as an insoluble protein cross-linked by interchain disulfide bonds forming high molecular mass polymers. It is abundant during embryonic development and tissue remodeling, and also present as a soluble plasma protein.
Fibronectin is composed of repeating modules forming domains that interact with other fibronectin molecules, matrix components (e.g., collagen, heparin), and cell receptors (e.g., integrins). It plays key roles in cell adhesion and migration processes such as embryogenesis, wound healing, blood coagulation, host defense, and metastasis.
Fibronectin expression is elevated in non-small cell lung carcinoma, where its interaction with cancer cells enhances tumorigenicity and resistance to apoptosis induced by chemotherapeutic agents. The gene encoding Fibronectin undergoes alternative splicing, potentially producing up to 20 transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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