Thermo Fisher Scientific Histone H3.3 Polyclonal Antibody
Histone H3.3 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot과 ELISA에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 핵 및 염색체 단백질 연구에 유용합니다. 액상 형태로 제공되며, -20°C에서 보관하십시오.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–136 of human H3F3A (NP_002098.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.48 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805587 |
Product Specific Information
Immunogen sequence:
MARTKQTARK STGGKAPRKQ LATKAARKSA PSTGGVKKPH RYRPGTVALR EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSAAI GALQEASEAY LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA
Positive Samples: U-87MG, HT-29, H460, HeLa, Mouse stomach, Mouse thymus, Rat stomach
Cellular Location: Chromosome, Nucleus
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of linker histone H1 with DNA between nucleosomes to form higher-order chromatin structures.
This gene encodes a member of the histone H3 family and is intronless. Its transcripts lack polyA tails and contain a palindromic termination element. The gene is located separately from other H3 genes within the histone gene cluster on chromosome 6p22-p21.3.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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