Norgen Plasmid MiniPrep 96-Well Kit (Magnetic Bead System)

Norgen Plasmid MiniPrep 96-Well Kit (Magnetic Bead System)

SKU: 63000
For rapid and convenient plasmid DNA preparations

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Features and Benefits

• Isolate high quality, high yield plasmid DNA
• Ready for downstream applications: restriction digestion, bacterial transformation, sequencing, and more
• Available formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), MaxiPrep

This kit enables rapid preparation of plasmid DNA from Escherichia coli cultures using magnetic bead technology. DNA binds under optimized salt conditions and is released under low salt, slightly alkaline conditions. Purified plasmids are fully digestible with all tested restriction enzymes and compatible with real-time PCR and NGS.
The 96-well format supports high-throughput automation.

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Kit Specifications

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Storage Conditions and Product Stability

All solutions should be tightly sealed and stored at room temperature.
Stable for 1 year from shipment date.
RNase should be stored at -20°C; Resuspension Solution A at 4°C after RNase addition.

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Kit Components

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Supporting Data

Figure 1. High DNA Yield from Small Cultures
Plasmid DNA from 1 mL overnight bacterial cultures was prepared with Norgen’s Plasmid MiniPrep Kit and compared with two other commercial kits.
Average yields:

Y-axis: Yield (micrograms)
X-axis: Norgen, Competitor 1, Competitor 2

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Figure 2. High-Accuracy Sequencing
One microgram of plasmid DNA purified by Norgen’s kit was used as a template in an Applied Biosystem DNA Sequencer.
Sequence accuracy: >99% over a 950 bp contiguous sequence.
Extracted sequence example:

C A G T T C A T T T T T G G C T T T G G A C T T T T T T T T T T T T T ...

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Figure 3. High Quality of Plasmid DNA
Plasmid DNA from E. coli (1 mL) was digested with EcoR I and analyzed on agarose gel.
All plasmid DNA samples were digestible, indicating high purity.

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Figure 4. High DNA Yield from 150 mL Cultures
Comparison of plasmid yields using Norgen’s MaxiPrep Kit and competitors.

Y-axis: Yield (micrograms)
X-axis: Norgen, Competitor 1, Competitor 2

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Figure 5. Full Compatibility with Digests
Plasmid DNA purified by Norgen’s MaxiPrep Kit was digested with various restriction enzymes.

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Documentation

• Protocol (Cat. 63000)
• Application Note: Plasmid DNA Purification Using Norgen Preparative Chromatography Columns
• Flyer: Microorganism - Solutions for Isolation and Downstream Applications

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FAQs

Why do I have poor DNA recovery?

Possible causes:

• Plasmid did not propagate (check growth medium and antibiotics)
• Old inoculum culture (use fresh colonies)
• Insufficient lysis (warm Lysis Buffer N)
• Incomplete resuspension (ensure homogeneity before adding Lysis Buffer)
• Incorrect elution buffer placement or pH

Why is purified DNA not performing well?

Possible causes:

• Incomplete washing (salt residues)
• Dry spin omitted (ethanol traces remain)
• Incorrect ethanol volume for wash buffer
• Incompatible elution buffer (high salts or detergents)

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Citations

Examples of studies using Norgen plasmid kits:

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Related Products

• HighRanger 1 kb DNA Ladder
• PCR Sizer 100 bp DNA Ladder
• PCR Ranger 100 bp DNA Ladder

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