ELK Biotechnology Smad2 (phospho Ser465) rabbit pAb
Smad2 (phospho Ser465)에 특이적인 rabbit polyclonal antibody로, TGF-β 신호전달 연구에 적합. WB, IHC, IF, ELISA에 사용 가능. 인산화된 Smad2 검출에 유용하며, 세포 내 위치 변화 연구에 활용 가능.
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제품명
Smad2 (phospho Ser465) rabbit pAb
제품 정보
| 항목 | 내용 |
|---|---|
| Alternative Names | SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2 |
| Applications | WB; IHC; IF; ELISA |
| Recommended Dilutions | Western Blot: 1/500–1/2000 Immunohistochemistry: 1/100–1/300 ELISA: 1/10000 Not yet tested in other applications |
| Immunogen | Synthesized phospho-peptide around the phosphorylation site of human Smad2 (phospho Ser465) |
| Host | Rabbit |
| Storage | -20°C / 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band | 52 kD |
| GeneID (Human) | 4087 |
| Human Swiss-Prot No. | Q15796 |
| Species Reactivity | Human; Mouse; Rat |
| Cellular Localization | Cytoplasm, Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696, PubMed:21145499). On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity). |
Background
The protein encoded by this gene belongs to the SMAD family, which is homologous to the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators mediating multiple signaling pathways. This protein mediates the signal of transforming growth factor (TGF)-beta and regulates cellular processes such as proliferation, apoptosis, and differentiation. It is recruited to TGF-beta receptors through interaction with SARA (SMAD anchor for receptor activation). Upon TGF-beta stimulation, the protein is phosphorylated, dissociates from SARA, and associates with SMAD4, enabling nuclear translocation and transcriptional regulation.
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