Thermo Fisher Scientific PHGDH Recombinant Rabbit Monoclonal Antibody (8H10Z3)
PHGDH 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, IHC, ICC, ELISA 등에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 세포에서 발현되었으며, 액상 형태로 -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:8,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:800 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 8H10Z3 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 264–533 of human PHGDH (NP_006614.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.08 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3094179 |
Product Specific Information
Immunogen sequence:
EEPPRDRALV DHENVISCPH LGASTKEAQS RCGEEIAVQF VDMVKGKSLT GVVNAQALTS AFSPHTKPWI GLAEALGTLM RAWAGSPKGT IQVITQGTSL KNAGNCLSPA VIVGLLKEAS KQADVNLVNA KLLVKEAGLN VTTSHSPAAP GEQGFGECLL AVALAGAPYQ AVGLVQGTTP VLQGLNGAVF RPEVPLRRDL PLLLFRTQTS DPAMLPTMIG LLAEAGVRLL SYQTSLVSDG ETWHVMGISS LLPSLEAWKQ HVTEAFQFHF
Target Information
The PHGDH gene encodes an enzyme involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been associated with congenital microcephaly, psychomotor retardation, and other symptoms. Multiple alternatively spliced transcript variants have been identified, though the full-length nature of most remains unknown.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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