Thermo Fisher Scientific PHGDH Recombinant Rabbit Monoclonal Antibody (8H10Z3)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:8,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:800

-

Immunocytochemistry (ICC/IF)

1:100-1:400

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8H10Z3

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PHGDH,uniProtId:O43175-1,ncbiNodeId:9606,antigenRange:264-533,antigenLength:533,antigenImageFileName:MA5-51365_PHGDH_O43175-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51365_PHGDH_O43175-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.08 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3094179

Product Specific Information

Immunogen sequence: EEPPRDRALV DHENVISCPH LGASTKEAQS RCGEEIAVQF VDMVKGKSLT GVVNAQALTS AFSPHTKPWI GLAEALGTLM RAWAGSPKGT IQVITQGTSL KNAGNCLSPA VIVGLLKEAS KQADVNLVNA KLLVKEAGLN VTTSHSPAAP GEQGFGECLL AVALAGAPYQ AVGLVQGTTP VLQGLNGAVF RPEVPLRRDL PLLLFRTQTS DPAMLPTMIG LLAEAGVRLL SYQTSLVSDG ETWHVMGISS LLPSLEAWKQ HVTEAFQFHF

Target Information

PHGDH gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.