Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), Alexa Fluor 700, eBioscience
인간 CD366(TIM3)을 인식하는 F38-2E2 단클론 항체로, Alexa Fluor 700으로 표지되어 있습니다. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 면역조절 관련 연구에 적합합니다. 4°C 암조건 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | – | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 7 publications |
| ELISA | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | F38-2E2 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation/Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784767 |
Available Formats:
Alexa Fluor 532, APC, APC-eFluor 780, Brilliant UV 805, Brilliant Violet 605/650/786, FITC, Functional Grade, PE, PE-Cyanine5/5.5/7, PE-eFluor 610, PerCP-eFluor 710, Super Bright 436/600/645/702/780.
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Product Specific Information
This F38-2E2 monoclonal antibody reacts with human CD366 (TIM3, HAVCR2), a cell surface receptor expressed on activated CD4⁺ T cell subsets (Th1, Th17, Treg), CD8⁺ T cells, monocytes, dendritic cells, and mast cells.
CD366 exists in membrane-bound and soluble forms due to alternative splicing, with Galectin-9 as its ligand. It negatively regulates CD4⁺ T cells and influences Th1/Th17 cytokine secretion. TIM3 is implicated in immune tolerance, autoimmune diseases (e.g., multiple sclerosis), and HIV infection.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry of stimulated human peripheral blood cells. Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).
Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited by the red laser (633–647 nm). Instruments typically require a 685 LP mirror and 710/20 band pass filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.
Target Information
TIM3 (HAVCR2) is a 281-amino-acid, type I transmembrane glycoprotein expressed on terminally differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells. It includes an IgV-like domain, a mucin-like extracellular domain, and a conserved cytoplasmic tyrosine phosphorylation motif.
TIM3 is involved in macrophage activation, autoimmune disease induction, and immune tolerance regulation. It down-regulates Th1-mediated immune responses and has been linked to experimental autoimmune encephalomyelitis (EAE) and immune pathogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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