Thermo Fisher Scientific CD366 (TIM3) Monoclonal Antibody (F38-2E2), Alexa Fluor 700, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats:
Alexa Fluor 532, APC, APC-eFluor 780, Brilliant UV 805, Brilliant Violet 605/650/786, FITC, Functional Grade, PE, PE-Cyanine5/5.5/7, PE-eFluor 610, PerCP-eFluor 710, Super Bright 436/600/645/702/780.
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Product Specific Information

This F38-2E2 monoclonal antibody reacts with human CD366 (TIM3, HAVCR2), a cell surface receptor expressed on activated CD4⁺ T cell subsets (Th1, Th17, Treg), CD8⁺ T cells, monocytes, dendritic cells, and mast cells.
CD366 exists in membrane-bound and soluble forms due to alternative splicing, with Galectin-9 as its ligand. It negatively regulates CD4⁺ T cells and influences Th1/Th17 cytokine secretion. TIM3 is implicated in immune tolerance, autoimmune diseases (e.g., multiple sclerosis), and HIV infection.

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry of stimulated human peripheral blood cells. Recommended usage: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Fluorochrome Information:
Alexa Fluor 700 emits at 723 nm and is excited by the red laser (633–647 nm). Instruments typically require a 685 LP mirror and 710/20 band pass filter.
Excitation: 633–647 nm; Emission: 723 nm; Laser: Red Laser.

Target Information

TIM3 (HAVCR2) is a 281-amino-acid, type I transmembrane glycoprotein expressed on terminally differentiated CD4⁺ Th1 and CD8⁺ Tc1 cells. It includes an IgV-like domain, a mucin-like extracellular domain, and a conserved cytoplasmic tyrosine phosphorylation motif.
TIM3 is involved in macrophage activation, autoimmune disease induction, and immune tolerance regulation. It down-regulates Th1-mediated immune responses and has been linked to experimental autoimmune encephalomyelitis (EAE) and immune pathogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.