Thermo Fisher Scientific VEGF Recombinant Rabbit Monoclonal Antibody (105)

Applications

• ELISA (ELISA)
  Tested Dilution: 1:5,000–1:10,000

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to avoid contamination.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.

Advantages include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire

This antibody has specificity for Human VEGF165/VEGFA.

Target Information

VEGF (vascular endothelial growth factor) is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis, promoting tumor progression and metastasis. It affects vascular endothelium by promoting viability, mitogenesis, chemotaxis, and permeability.

The VEGF family includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system (FLT1/VEGFR-1 and FLK1/KDR/VEGFR-2) are fundamental regulators of angiogenesis signaling.

Five isoforms of VEGF are produced via alternative splicing of a single gene. A soluble form of FLT1 (sFLT1) binds VEGF with high affinity, inhibiting angiogenesis.

Elevated VEGF levels are associated with POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes), also known as Crow-Fukase syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.