Thermo Fisher Scientific ROR1 Monoclonal Antibody (2H6)

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:200–1:1,000

ELISA

• Tested Dilution: 1:10,000

Product Specifications

Product Specific Information

MA5-15539 targets ROR1 in indirect ELISA, IF, and WB applications and shows reactivity with human samples.
The immunogen is a recombinant extracellular fragment of human ROR1 (aa30–406) fused with hIgGFc tag, expressed in HEK293 cells.
MA5-15539 detects ROR1, which has a predicted molecular weight of approximately 101 kDa.

Target Information

Receptor-tyrosine-kinase-like orphan receptor 1 (ROR1) is a tumor-associated surface protein predominantly expressed during embryogenesis, involved in organ morphogenesis, nervous system development, and neural progenitor cell maintenance and survival.
It is virtually absent from normal pediatric and adult tissues except for low-level expression in hematogones and adipocytes.
ROR1 is notably overexpressed and considered a survival factor in several B lymphoid and epithelial malignancies, including chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), acute lymphoblastic leukemia (ALL), marginal zone lymphoma, and lung adenocarcinoma.
Wnt-5a has been suggested as a candidate ligand for ROR1, which may function as a pseudokinase promoting proliferation and resistance to apoptosis in cancer cells through interaction with Wnt-5a and TCL1-mediated AKT activation.
ROR1 is expressed as a glycoprotein containing extracellular immunoglobulin-like, Frizzled, and Kringle domains, as well as an intracellular tyrosine kinase domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.