Thermo Fisher Scientific RGMA Recombinant Rabbit Monoclonal Antibody (2S1B4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2S1B4

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human RGMA (Q96B86). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:RGMA,uniProtId:Q96B86-1,ncbiNodeId:9606,antigenRange:350-450,antigenLength:450,antigenImageFileName:MA5-42630_RGMA_Q96B86-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42630_RGMA_Q96B86-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.67 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911771

Product Specific Information

Positive test controls include: Mouse heart, Rat skeletal muscle,Rat heart. The target is usually found in the following locations: Cell membrane, GPI-anchor, Lipid-anchor.

Immunogen sequence: TAPETFPYET AVAKCKEKLP VEDLYYQACV FDLLTTGDVN FTLAAYYALE DVKMLHSNKD KLHLYERTRD LPGRAAAGLP LAPRPLLGAL VPLLALLPVF C

Target Information

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.