Thermo Fisher Scientific ALKBH1 Monoclonal Antibody (OTI4C4), TrueMAB
인간 ALKBH1 단백질(aa 1-245)에 특이적인 Mouse IgG1 단일클론 항체로, IHC(P)에서 1:150 희석으로 사용 가능. 무보존제, 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도 유지. DNA 손상 복구 연구용으로 적합.
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Thermo Fisher Scientific ALKBH1 Monoclonal Antibody (OTI4C4), TrueMAB
Applications and Tested Dilution
- Immunohistochemistry (Paraffin) [IHC (P)]: 1:150
Publications
- [References not provided]
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4C4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–245 of human ALKBH1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The ALKBH1 gene encodes a homolog of the E. coli alkB protein, which participates in DNA alkylation damage repair through oxidative demethylation.
This dioxygenase repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation and requires molecular oxygen, alpha-ketoglutarate, and iron.
ALKBH1 may play a role in placental trophoblast lineage differentiation and exhibits DNA lyase activity, introducing double-stranded breaks at abasic sites. It cleaves both single- and double-stranded DNA, showing highest activity on double-stranded DNA with two abasic sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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