Thermo Fisher Scientific Estrogen Receptor alpha Monoclonal Antibody (6F11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 24 publications 24 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:40-1:80

View 8 publications 8 publications

Immunohistochemistry (Frozen) (IHC (F))

1:40-1:60

-

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

ChIP assay (ChIP)

5 µL/10^6 cells

-

Gel Shift (GS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Cat, Human, Mouse, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6F11

Immunogen

Recombinant estrogen receptor protein (alpha form)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

purified

Storage buffer

tissue culture supernatant

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_780508

Product Specific Information

Paraffin sections require an antigen retrieval pretreatment with sodium citrate buffer (pH 6.0) prior to staining. Use Samboni`s fixative for optimal staining results. Positive control of Breast carcinoma suggested.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.