Applied Biosystems™

SYBR™ Green Universal Master Mix

Everything you need for SYBR™ Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems™ SYBR™ Green자세히 알아보기

Everything you need for SYBR™ Green dye–based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems™ SYBR™ Green PCR Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.

• Premixed components stored at 2–8°C significantly reduce assay setup time
• SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability

Maximum Flexibility and Convenience
Applied Biosystems™ SYBR™ Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required. SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.

Alternative product
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we`ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.

사양

검출 방법SYBR

형식Tube

GC-Rich PCR PerformanceHigh

PCR 방법qPCR

중합효소AmpliTaq Gold DNA Polymerase

반응 속도Standard

용도(장비)7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System

라벨 또는 염료SYBR Green

패시브 참조 염료ROX (Pre-mixed)

제품라인SYBR™

제품 유형Real Time PCR SYBR Master Mix

수량1 x 5 mL

배송 조건Wet Ice

충분200 Reactions of 50 μL

용도(애플리케이션)Gene Expression

농도2X

반응 수200 Reactions

샘플 종류DNA (Genomic), cDNA

부피1 x 5 mL

Unit SizeEach