Thermo Fisher Scientific N-Cadherin/Cadherin-2/CD325 (NCAD) Monoclonal Antibody (MNCD2)
N-Cadherin(CD325) 단백질을 인식하는 MNCD2 클론의 Rat 모노클로날 항체. Western blot, IHC, Flow cytometry 등 다양한 응용에 적합. 고순도 Protein A/G 정제, PBS/BSA buffer로 안정적 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific N-Cadherin/Cadherin-2/CD325 (NCAD) Monoclonal Antibody (MNCD2)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MNCD2 |
| Immunogen | Recombinant fragment (aa308–597) around mouse CDH2. The epitope is the extracellular domain of N-cadherin. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
N-cadherin is a 140 kDa transmembrane protein that mediates calcium-dependent intercellular adhesion. It belongs to the cadherin family, which regulates morphogenetic movements and cell surface adhesion through homotypic interactions. N-cadherin’s function depends on its association with the actin cytoskeleton via interactions with cytoplasmic catenin proteins. This association is regulated by phosphorylation and dephosphorylation of beta-catenin. N-cadherin is a calcium-dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It plays a crucial role during gastrulation and in establishing left-right asymmetry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송 정보
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