Thermo Fisher Scientific HDAC4 Polyclonal Antibody
HDAC4 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, IP, ChIP 등 다양한 응용에 사용 가능. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며 장기 보관 시 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
| ChIP assay (ChIP) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 363–639 of Human HDAC4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.0, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2546579 |
Product Specific Information
- Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2
- Predicted reactivity: Mouse (90%), Rat (88%), Chicken (84%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Histone deacetylase 4 (HDAC4) is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation provides a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. HDAC4 acts via the formation of large multiprotein complexes and is involved in muscle maturation through interactions with myocyte enhancer factors (MEF2A, MEF2C, MEF2D). It also participates in MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Mutations in the HDAC4 gene have been associated with Brachydactyly-mental retardation syndrome (BDMR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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