Thermo Fisher Scientific eIF3f Polyclonal Antibody
Thermo Fisher Scientific의 eIF3f Polyclonal Antibody는 인간 eIF3f 단백질을 인식하는 토끼 다클론 항체로, Western blot 및 IHC(P) 분석에 적합합니다. 높은 특이성과 재현성을 제공하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.04–0.4 µg/mL
Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 1:200–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human eIF3f. Recombinant protein control fragment (Product # RP-100070) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2640964 |
Product Specific Information
Immunogen sequence:
DSYERRNEGA ARVIGTLLGT VDKHSVEVTN CFSVPHNESE DEVAVDMEFA KNMYELHKKV SPNELILGWY A
- Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 100%
Target Information
The eIF3f protein is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, essential for multiple steps in the initiation of protein synthesis.
The eIF-3 complex associates with the 40S ribosome and facilitates recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of mRNA for AUG recognition.
Additionally, eIF-3 is required for disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of 40S and 60S subunits before initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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