Thermo Fisher Scientific IL-22 Receptor alpha Monoclonal Antibody (496514)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

8-25 µg/mL

-

Flow Cytometry (Flow)

2.5 µg/1x10^6 cells

-

Product Specifications

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

496514

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-22 R alpha 1 Thr18-Ala228

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2607024

Product Specific Information

In direct ELISAs, 25% cross-reactivity with recombinant human (rh) IL-22 R alpha 1 is observed and no cross-reactivity with rhIL-20 R alpha, recombinant mouse (rm) IL-20 R alpha, rhIL-22BP, or rmIL-22BP is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

A novel cytokine, designated IL-TIF for IL-10 related T cell-derived inducible factor and IL-22, was recently identified. The receptor for IL-22 (IL-22R, also termed CRF2-9 and IL-TIF-R1 chain) is a new member of the class II cytokine receptor family. IL-22R forms a complex with IL-10 receptor beta chain and mediates IL-22 signaling. IL-22 and its receptor activate JAK-STAT signaling pathway. IL22R is expressed in normal liver and kidney and their cell lines HepG2 and TK-10. A soluble form of IL-22 receptor, also termed IL-22 binding protein (IL-22BP) and IL-22 receptor-alpha 2 (IL-22RA2), was identified very recently. IL-22BP prevents binding of IL-22 to the functional cell surface IL-22R complex and neutralizes IL-22 activity. LPS induces IL-22 expression, which indicates the role of IL-22 in inflammatory response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.