Thermo Fisher Scientific IL12 p35 Polyclonal Antibody, FITC
FITC로 표지된 IL12 p35 폴리클로날 항체로 인간 및 비인간 영장류 반응성. WB, ELISA, IP 등 다양한 응용 가능. 고순도 친화크로마토그래피 정제, 안정한 액상 형태로 보관 용이. 연구용 시약으로 세포 신호 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 210–260 of human IL12P35 subunit protein |
| Conjugate | FITC (Fluorescein) |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Interleukin 12A (IL-12A) is a signaling cytokine belonging to the Interleukin-6 protein superfamily. The IL-12A alpha chain precursor (IL-12A) forms a 35-kD subunit of the disulfide-linked heterodimer IL-12 and exhibits diverse biological activities.
IL-12A acts as a growth factor for activated T and NK cells, enhancing NK/lymphokine-activated killer cell activity. It stimulates IFN-γ production by resting PBMCs and supports differentiation of Th1 and Th2 cells.
Additionally, IL-12A positively regulates tyrosine phosphorylation of Stat4 protein and participates in innate immunity signaling requiring nitric oxide synthase 2A.
Together with EBI3, IL-12A forms interleukin-35 (IL-35), a heterodimeric cytokine produced by Treg cells, essential for maximal suppressive activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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