Thermo Fisher Scientific ATF6 Polyclonal Antibody

Applications

• Western Blot (WB): Assay-dependent

Product Specifications

Product Specific Information

• Keep as concentrated solution.
• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3' half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE.
ATF6 may also be involved in activation of transcription by the serum response factor. It exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC), and also interacts with transcription factors GTF2I, YY1, and SRF.
Under ER stress, the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal, and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response, an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis, sequentially cleaved by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK, and belongs to the bZIP family.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.