Thermo Fisher Scientific Phospho-Huntington (Ser421) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Huntingtin pS421 is directed against the phosphorylated form of the S421 residue. A BLAST analysis suggests cross-reactivity with human based on 100% sequence homology. Cross-reactivity with Huntington pS421 from other sources has not been determined.

Target Information

Huntingtin is a disease gene linked to Huntington’s disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product.

HD is a mid-life onset autosomal dominant neurodegenerative disease characterized by psychiatric disorders, dementia, and involuntary movements (chorea), leading to death in 10–20 years. The huntingtin locus spans 180 kb and consists of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as two alternatively polyadenylated forms with different relative abundance in various fetal and adult tissues. The larger transcript (~13.7 kb) is expressed predominantly in adult and fetal brain, while the smaller transcript (~10.3 kb) is more widely expressed.

The genetic defect leading to Huntington’s disease may not eliminate transcription but may confer a new property on the mRNA or alter the function of the protein.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.