Thermo Fisher Scientific PI3K p85 alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Protein corresonding to Human PI3K p85 alpha. Recombinant protein control fragment (Product #RP-95921). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PI3K p85 alpha,uniProtId:P27986-1,ncbiNodeId:9606,antigenRange:182-328,antigenLength:724,antigenImageFileName:PA5-110625_PI3K_p85_alpha_P27986-1_Rabbit.svg,antigenImageFileNamePDP:PA5-110625_PI3K_p85_alpha_P27986-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2856036

Product Specific Information

Immunogen sequence: LADAFKRYLL DLPNPVIPAA VYSEMISLAP EVQSSEEYIQ LLKKLIRSPS IPHQYWLTLQ YLLKHFFKLS QTSSKNLLNA RVLSEIFSPM LFRFSAASSD NTENLIKVIE ILISTEWNER QPAPALPPKP PKPTTVANNG MNNNMSL

Target Information

Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.