Thermo Scientific™

Ion 16S™ Metagenomics Kit

The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive and broad-range analyses of mixed microbial populations using the Ion자세히 알아보기

The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive and broad-range analyses of mixed microbial populations using the Ion Torrent™ semiconducter sequencing workflow. The kit permits PCR amplification of hypervariable regions of the 16S rDNA gene from bacteria. The amplified fragments can then be sequenced using the Ion PGM™ Sequencing 400 Kit on the Ion PGM™ platform and analyzed using the Ion 16S™ metagenomics analyses module within the Ion Reporter™ software.

Comprehensive

The kit includes 2 sets of primers that can be used to amplify the corresponding hypervariable regions of the 16S rDNA gene in bacteria:

• Primer set V2-4-8
• Primer set V3-6,7-9

These comprehensive primer sets allow for accurate detection and identification of a broad range of bacteria down to the genus or species level. The primer sets are paired with Environmental Master Mix v2.0 that is optimized to tolerate high levels of PCR inhibitors and amplify targets from complex samples such as environmental, food, tissue and other challenging samples. Additionally, the kit includes a positive control for troubleshooting experiments.

Flexible

The primer sets are provided as two pools. Each pool can be used individually or in combination to allow for flexibility in experimental set up. When combined with the Ion Xpress™ Barcode Adapters kits, multiple polymicrobial samples can be combined in a single sequencing workflow.

Complete Workflow

Sequences generated using the Ion 16S™ Metagenomics Kit can be directly analyzed using the Ion 16S™ metagenomics analyses module within the Ion Reporter™ software enabling a rapid and semi-quantitative assessment of complex microbial samples.

Note: This kit is not compatible with the Ion Library Quantitation Kit (Cat. No. 4468802). For accurate quantitation of amplicons generated using this kit, use the Ion Universal Library Quantitation Kit (Cat. No. A26217).

사양

검출 방법Ion Torrent Sequencing, Primer-Probe Detection

샘플 종류DNA

용도(장비)Ion GeneStudio™ S5 Series Sequencer

용도(애플리케이션)Sequencing

반응 수250 Reactions

중합효소Taq DNA Polymerase

제품라인Ion Torrent™

수량100 reactions

종All Species

타겟 유기체 분류All Bacteria

테스트 시간Fast

Unit SizeEach