Thermo Fisher Scientific CD43 Recombinant Mouse Monoclonal Antibody (rSPN, 839)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• For immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with a high negative charge, expressed on the surface of most hematopoietic cells.
It contributes to a repulsive barrier that interferes with cellular adhesion but can also promote leukocyte aggregation in certain contexts.
Through interactions with actin-binding proteins such as ezrin and moesin, CD43 regulates T-cell morphology and cell-cell interactions during lymphocyte trafficking.
CD43 signaling enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors.
Expression of CD43 can induce functionally active tumor suppressor p53 protein, but in the absence of p53 and ARF, CD43 promotes tumor proliferation and viability.
Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.