Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (eBioC9B7W (C9B7W)), PE-Cyanine7

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 1 µg/test
  View 11 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Brilliant Violet 480
• eFluor 450
• FITC
• Functional Grade
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Request custom conjugation

Product Specific Information

The eBioC9B7W monoclonal antibody recognizes mouse CD223 (LAG-3, LAG3) protein expressed by activated α/β-TCR bearing T cells, a subset of γ/δ-TCR bearing T cells, and NK cells.
CD223 is a 70 kDa type I transmembrane protein similar to CD4. Soluble forms have been detected, resulting in a 54 kDa extracellular fragment and a 16 kDa intracellular fragment.
It binds MHC class II with higher affinity than CD4, contributing to negative regulation of T-cell activation and proliferation. CD223 is also expressed on CD4+CD25+ regulatory T cells.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on stimulated mouse splenocytes at ≤1 µg/test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test.
Antibody titration recommended for optimal performance.

Light Sensitivity: Protect from light; tandem dye is photo-sensitive.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) for up to 3 days at 4°C in the dark.
Excitation: 488–561 nm
Emission: 775 nm
Laser: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

LAG-3 (CD223) is a 70 kDa surface glycoprotein of the Ig superfamily, homologous to CD4. It binds MHC class II with higher affinity than CD4 and plays a role in downregulating T-cell activation and proliferation.
Expressed on activated T cells, regulatory T cells, and NK cells. CD8+ T cells typically express higher levels than CD4+ T cells.
Coexpression with CD49b identifies Type 1 regulatory T cells (Tr1) in human and mouse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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