Thermo Fisher Scientific Selectin E Polyclonal Antibody
Thermo Fisher Scientific의 Selectin E Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하는 Rabbit IgG 폴리클로날 항체입니다. Western blot 및 IHC(P) 실험에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 염증 반응에서 E-selectin 발현 연구에 활용됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 0.1–1 µg/mL
Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 2–10 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the N-terminus (from 35aa–80aa) of human Selectin E protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Jurkat Cells
- Cellular location: Membrane
Target Information
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. Leukocyte adhesion also occurs in capillaries and small venules throughout the body after inflammation onset. This process involves a cascade of adhesion receptors, including selectins and members of the immunoglobulin superfamily.
E-selectin (CD62E) is an endothelial cell-specific selectin expressed only after activation by proinflammatory cytokines such as IL-1, TNFα, and bacterial wall components like lipopolysaccharides. These stimuli induce E-selectin transcription via an NFκB-dependent signaling cascade. E-selectin is associated with endothelial cells in various inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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