Thermo Fisher Scientific HMGB1 Recombinant Rabbit Monoclonal Antibody (7H38L51)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody is predicted to react with Bovine.

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. Recent studies indicate that damaged or necrotic cells release HMGB1 into the extracellular environment, where it functions as a proinflammatory cytokine.

Mouse HMGB1 is a 215 amino acid single-chain polypeptide containing three domains:

• Two tandem-linked positively charged DNA-binding domains: HMG box A (aa 9–79) and box B (aa 89–162)
• A negatively charged 30 aa C-terminal acidic tail region

Residues 28–44 and 180–185 contain a nuclear localization signal (NLS).
The cytokine activity resides in the B box, while the A box associates with the helix-loop-helix domain of transcription factors.

HMGB1 acts as:

• An inflammatory mediator promoting monocyte migration and cytokine secretion
• A mediator of T cell–dendritic cell interaction
• A ligand for receptors such as RAGE, TLR2, and TLR4

HMGB1 is highly conserved and ubiquitously expressed in nuclei and cytoplasm of nearly all cell types. It is a key mediator of inflammation in sterile and infection-associated responses and functions as a nuclear DNA-binding protein. Elevated HMGB1 levels are reported during sterile tissue injury, infection, endotoxemia, sepsis, arthritis, and ischemia-reperfusion injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.