Thermo Fisher Scientific ROBO1 Recombinant Mouse Monoclonal Antibody (10E2-R)
ROBO1 단백질을 인식하는 재조합 마우스 단클론 항체로, WB 및 IHC(P) 분석에 최적화됨. 인간, 마우스, 랫트 반응성. HEK293 발현 시스템 기반, Protein A 정제. PBS 버퍼에 보관하며 장기 저장 시 -20°C 권장.
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Thermo Fisher Scientific ROBO1 Recombinant Mouse Monoclonal Antibody (10E2-R)
Applications and Tested Dilution
- Western Blot (WB): 1:1,000
- Immunohistochemistry (Paraffin) (IHC (P)): 1:200–1:600
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 10E2-R |
| Immunogen | Recombinant protein within Human ROBO1 aa 51–300/1651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3094248 |
Product Specific Information
- Positive Controls: MCF-7 cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, PC-12 cell lysate, RAW264.7 cell lysate, human breast tissue, human placenta tissue
- Predicted Band Size: 181 kDa
- Subcellular Location: Cell membrane, Cell projection (axon), Endoplasmic reticulum-Golgi intermediate compartment membrane
Target Information
Specialized cells at the midline, which separates the left and right halves of the central nervous system, play key roles in directing growth cone behavior. In vertebrates and invertebrates, midline cells produce guidance cues such as nectins and slit, acting as attractants and repellents. Robo proteins serve as axon guidance receptors and belong to a conserved subfamily of Ig superfamily proteins. Robo1 acts as a receptor for the repellent Slit and functions cell-autonomously. Non-crossing axons express high levels of Robo, while crossing axons modulate expression before and after midline crossing. Robo1 and Robo2 are human homologs of the Drosophila Roundabout protein; Robo1 is also homologous to the C. elegans sax3 gene, and Robo2 to the zebrafish astray gene.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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