Thermo Fisher Scientific ROBO1 Recombinant Mouse Monoclonal Antibody (10E2-R)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:600

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

10E2-R

Immunogen

Recombinant protein within Human ROBO1 aa 51-300/1651. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ROBO1,uniProtId:Q9Y6N7-1,ncbiNodeId:9606,antigenRange:51-300,antigenLength:1651,antigenImageFileName:MA5-50631_ROBO1_Q9Y6N7-1_House_mouse.svg,antigenImageFileNamePDP:MA5-50631_ROBO1_Q9Y6N7-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3094248

Product Specific Information

Positive control: MCF-7 cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, PC-12 cell lysate, RAW264.7 cell lysate, human breast tissue, human placenta tissue.

Predicted band size: 181 kDa

Subcellular Location: Cell membrane, Cell projection, axon, Endoplasmic reticulum-Golgi intermediate compartment membrane.

Target Information

Specialized cells at the midline, which separates the left and right halves of the CNS, have a number of roles in directing growth cone behavior. In the vertebrate spinal cord, the insect ventral nerve cord and in C. elegans, midline cells produce guidance cues such as nectins and slit, which act as attractants and repellents, respectively. These cells may act as gatekeepers to prevent axons from crossing the midline and to induce a switch in growth cone responsiveness to guidance cues beyond the gateway. One such gatekeeper, Robo, is an axon guidance receptor that defines a novel subfamily of Ig superfamily proteins that are conserved from fruit flies to mammals. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous fashion. Non-crossing axons express high levels of Robo, whereas crossing axons express low levels of Robo before reaching the midline and high levels after they cross. Robo1 and Robo2 are two human homologs of the Drosophila protein Roundabout. Robo1 is also homologous to the C. elegans gene sax3, whereas Robo2 is homologous to the zebrafish gene astray.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.