Thermo Fisher Scientific PHD4 Polyclonal Antibody
Thermo Fisher Scientific의 PHD4 Polyclonal Antibody는 인간 Prolyl Hydroxylase Domain-Containing Protein 4을 인식하는 Rabbit IgG 기반 항체입니다. Western blot에 최적화되어 있으며, 세포 산소 감지 및 HIF-alpha 연구에 활용됩니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Thermo Fisher Scientific PHD4 Polyclonal Antibody
Applications
- Western Blot (WB)
Tested Dilution
- 1:500–1:2,500
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 450 and the C-terminus (residue 502) of human Prolyl Hydroxylase Domain-Containing Protein 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
Target Information
Prolyl hydroxylase 4 (PHD4) is an enzyme that modifies HIF-alpha, playing a critical role in oxygen homeostasis. Unlike collagen-modifying prolyl hydroxylases found in the endoplasmic reticulum, HIF-related hydroxylases act intracellularly.
Under normoxic conditions, PHD4 hydroxylates specific prolyl residues in HIF-alpha, leading to its degradation via the von Hippel-Lindau ubiquitylation complex.
Under hypoxia, hydroxylation is reduced, stabilizing HIF-alpha and promoting angiogenesis. PHD4 functions as an oxygen sensor and catalyzes the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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