Thermo Fisher Scientific p14ARF Monoclonal Antibody (4C6/4)
인간 및 랫트 반응성을 가진 p14ARF 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 실험에 사용 가능. 단백질 A/G 정제, PBS 완충액 보존, -20°C에서 안정적 보관. 종양억제 단백질 연구용으로 적합.
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Thermo Fisher Scientific p14ARF Monoclonal Antibody (4C6/4)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4C6/4 |
| Immunogen | Recombinant full-length human p14ARF protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The INK4a locus on chromosome 9p21 is frequently affected in human tumors. It encodes two structurally distinct tumor suppressor proteins, p16INK4a and the alternative reading frame protein, ARF (p14ARF in human and P19ARF in mouse). These proteins interact with upstream tumor suppressors, retinoblastoma protein and p53, respectively. Each plays a role in cellular senescence and cell cycle control. p14ARF (or P19ARF in mouse) can induce G1 and G2 phase arrest dependent on functional p53. The functional differences between mouse and human ARF proteins may contribute to species-specific tumor susceptibility.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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