Thermo Fisher Scientific P4HA1 Polyclonal Antibody
Human P4HA1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 0.2 mg/mL 농도의 액상 형태로 제공됩니다. 콜라겐 합성 관련 연구용으로 최적화된 항체입니다.
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Thermo Fisher Scientific P4HA1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human P4HA1 (Product #RP-89690) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Wet ice |
| RRID | AB_2645146 |
Product Specific Information
Immunogen sequence:
FTSIGQMTDL IHTEKDLVTS LKDYIKAEED KLEQIKKWAE KLDRLTSTAT KDPEGFVGHP VNAFKLMKRL NTEWSELENL VLKDMSDGFI SNLTIQRQYF PNDEDQVGAA KALLRLQDTY NLDTDTISKG NLPGVKHKSF LTAEDC
Antigen sequence identity:
- Mouse: 97%
- Rat: 97%
Target Information
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline, essential for proper folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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