Thermo Fisher Scientific Calcineurin B Monoclonal Antibody (212306)
Calcineurin B 단백질을 인식하는 Mouse monoclonal 항체로, Human, Mouse, Rat 시료에서 사용 가능. Western blot에 최적화되어 있으며, 단백질 인산화 조절 연구에 유용. Lyophilized 형태로 제공되며, 재구성 후 0.5 mg/mL 농도로 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 0.5 µg/mL
- View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 212306 |
| Immunogen | E. coli-derived recombinant human Calcineurin B (Met1–Val170) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2608338 |
Product Specific Information
This antibody detects endogenous human, mouse, and rat Calcineurin B.
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Calcineurin is a Ser/Thr, calcium and calmodulin-dependent protein phosphatase essential for T cell activation. It consists of two subunits:
- Calcineurin A (CnA): 60 kDa catalytic subunit
- Calcineurin B (CnB): 19 kDa regulatory subunit containing four EF-hand calcium-binding sites
Calcineurin dephosphorylates the nuclear factor of activated T-cells (NF-AT), enabling its activation and nuclear translocation. Immunosuppressive drugs such as cyclosporin A and FK506 inhibit this process by forming complexes that block substrate access to the catalytic domain of CnA without affecting phosphatase activity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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