Thermo Fisher Scientific PKM Monoclonal Antibody (4C8E12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000-1:512,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:150-1:300

-

Flow Cytometry (Flow)

1:50-1:100

-

Immunoprecipitation (IP)

1-4 µL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

4C8E12

Immunogen

Recombinant Human Pyruvate kinase PKM protein (2-531aa) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKM1/PKM2,uniProtId:P14618-1,ncbiNodeId:9606,antigenRange:2-531,antigenLength:531,antigenImageFileName:MA5-38279_PKM1PKM2_P14618-1_House_mouse.svg,antigenImageFileNamePDP:MA5-38279_PKM1PKM2_P14618-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

4.31 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% ProClin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_2898194

Product Specific Information

This antibody has been tested in direct-ELISA

Target Information

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.