Thermo Fisher Scientific CD4 Monoclonal Antibody (10C12)

Applications

Tested Dilution

Publications

Western Blot (WB)

5-10 µg/mL

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5-10 µg/mL

-

ELISA (ELISA)

10-20 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10C12

Immunogen

Synthetic peptide corresponding to residues L S E K K T C Q C P H R F Q K T C S P I of human CD4 (aa 440-458). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD4,uniProtId:P01730-1,ncbiNodeId:9606,antigenRange:440-458,antigenLength:458,antigenImageFileName:MA1-10800_CD4_P01730-1_House_mouse.svg,antigenImageFileNamePDP:MA1-10800_CD4_P01730-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.55 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1072330

Product Specific Information

MA1-10800 detects CD4 Cytoplasmic Tail from human samples.

MA1-10800 has been successfully used in ELISA, immunofluorescence, and Western blot applications.

The MA1-10800 immunogen is a synthetic peptide corresponding to residues L S E K K T C Q C P H R F Q K T C S P I of human CD4.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.