Thermo Fisher Scientific TAP Tag Monoclonal Antibody (22F12-1E3)
TAP 태그 단백질 검출용 마우스 단클론 항체로, Western blot 및 IP에 적합합니다. TEV 절단 후 TAP C-말단 펩타이드로 면역화되었습니다. 비접합 액상 형태로, Protein A 정제 및 BSA 함유 PBS 버퍼에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 22F12-1E3 |
| Immunogen | KLH-conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536814 |
Available Conjugate Formats
Product Specific Information
MA1-108 detects TAP in Western blot applications. The immunogen is a KLH-conjugated peptide representing the C-terminus of the TAP construct after TEV cleavage. TAP adds about 20 kDa to the size of the protein.
Target Information
Epitope tags provide a method to localize gene products in various cell types, study protein topology and complexes, identify associated proteins, and characterize newly identified or low-abundance proteins when specific antibodies are not available.
Tandem affinity purification (TAP) is an affinity purification method for isolating TAP-tagged proteins along with associated proteins. The TAP tag typically includes a calmodulin binding peptide (CBP), a TEV protease cleavage site, and Protein A. This technique is useful for analyzing in vivo protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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