
Thermo Fisher Scientific Phospho-MEK1 (Thr386) Polyclonal Antibody
Phospho-MEK1 (Thr386) 폴리클로날 항체로, 인간·마우스·랫트 시료에서 MEK1 인산화 상태 분석에 적합합니다. WB, IHC, ICC, ELISA 등 다양한 응용에 사용 가능하며, 단백질 A 정제 및 -20°C 보관 조건을 갖춘 고품질 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Thr386 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in mitogen-activated protein kinase (MAPK) cascades, regulating cell growth and differentiation. It is activated by various growth factors, cytokines, and calcium influx, and phosphorylated by Raf1 at serine 218 and 222 residues. MEK1 is involved in stress response, apoptosis, and cytokine-induced proliferation. Mutations in the MAP2K1 gene are associated with cardiofaciocutaneous syndrome.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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