Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), Super Bright 702, eBioscience
CD185(CXCR5) 단백질을 인식하는 MU5UBEE 단클론 항체로, Super Bright 702 형광체가 결합된 제품입니다. 인간 및 비인간 영장류 시료에 적용 가능하며, 주로 유세포분석(Flow Cytometry)에 사용됩니다. 405 nm 레이저로 여기되며, 702 nm 파장에서 방출됩니다. 4°C에서 암소 보관하십시오.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | 8 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Human, Rhesus monkey |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright™ 702 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MU5UBEE |
| Immunogen | N-terminal extracellular domain of CXCR5 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2717182 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 532, 700
- APC, Biotin
- Brilliant UV 395, 615, 737, 805
- Brilliant Violet 421, 480, 605, 786
- eFluor 450, FITC
- NovaFluor Blue, Red, UV, Violet, Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645
- Custom conjugation available
Product Specific Information
The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185 (CXCR5/BLR1), a seven-transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma.
CD185 is expressed on B cells, CD4+ T cells, and a subset of memory T cells. It plays a key role in lymphocyte trafficking, particularly T cell migration into B cell follicles in response to CXCL13, making it an established marker of follicular helper T cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.5 µg) per test.
Fluorochrome Information:
Super Bright 702 is a tandem dye excited by the violet laser (405 nm) and emits at 702 nm. Recommended detection filter: 710/50 bandpass.
Staining Considerations:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Fixation:
Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Light Sensitivity:
Protect from light to prevent photo-induced oxidation.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
Target: CXCR5 (C-X-C Chemokine Receptor Type 5, CD185, BLR1)
CXCR5 is a G protein-coupled receptor for chemokine BLC, expressed in Burkitt’s lymphoma and lymphatic tissues such as lymph node follicles and spleen. It plays an essential role in B cell migration and lymphocyte localization within germinal centers.
CXCR5 expression has been observed in various tissues including blood, bone marrow, brain, liver, lymph node, spleen, and tonsil. As a GPCR family member, it participates in complex signaling and regulation pathways, making it a significant research target for immunology and pharmacology.
For Research Use Only. Not for use in diagnostic procedures.
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