Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, FITC, eBioscience

Applications

Tested Dilution

Immunohistochemistry (IHC)

Assay-Dependent

Immunocytochemistry (ICC/IF)

1 µg/mL

Flow Cytometry (Flow)

1 µg/test

Product Specifications

Species Reactivity

Rabbit

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

FITC FITC FITC

View additional formats

• [Alexa Fluor 488-10 nm colloidal gold](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 488-10 nm colloidal gold/host/Goat/)
• [Alexa Fluor 488-5 nm colloidal gold](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 488-5 nm colloidal gold/host/Goat/)
• [Alexa Fluor 568 ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Alexa Fluor 568/host/Goat/)
• [AP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Biotin/host/Goat/)
• [DyLight 350](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 350/host/Goat/)
• [DyLight 405](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 405/host/Goat/)
• [DyLight 488](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 488/host/Goat/)
• [DyLight 550](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 550/host/Goat/)
• [DyLight 594](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 594/host/Goat/)
• [DyLight 633](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 633/host/Goat/)
• [DyLight 650](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 650/host/Goat/)
• [DyLight 680](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 680/host/Goat/)
• [DyLight 800 4X PEG](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/DyLight 800 4X PEG/host/Goat/)
• [HRP](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/HRP/host/Goat/)
• [NANOGOLD](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/NANOGOLD/host/Goat/)
• [PE ](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/PE/host/Goat/)
• [PE-Cyanine5.5](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/PE-Cyanine5.5/host/Goat/)
• [Qdot 525](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 525/host/Goat/)
• [Qdot 585](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 585/host/Goat/)
• [Qdot 605](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 605/host/Goat/)
• [Qdot 625](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 625/host/Goat/)
• [Qdot 655](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 655/host/Goat/)
• [Qdot 705](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 705/host/Goat/)
• [Qdot 800](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/Qdot 800/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/TRITC/host/Goat/)
• [WesternDot 625](/antibody/secondary/product-name/Rabbit IgG (H--L)/conjugates/WesternDot 625/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1210845

Target

IgG

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Description: The anti-rabbit IgG polyclonal antibody reacts with the heavy and light chains of rabbit IgG, with minimal cross reaction to human, mouse and rat serum proteins. Reactions to non-immunoglobulin rabbit serum proteins have not been detected.

Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.

Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis and immunocytochemistry to detect polyclonal rabbit antibodies. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For immunocytochemistry applications, this can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.