Thermo Fisher Scientific GBL Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100

Product Specifications

Product Specific Information

• Positive Control: 293T, A431, HeLa, HepG2
• Predicted Reactivity: Zebrafish (91%), Xenopus laevis (90%), Bovine (98%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

G-protein beta subunit-like (GBL protein) is a novel member of the WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs. LST8 is a functional component of the mTOR signaling complex (TORC1) and positively regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 participates in regulating cell growth and nutrient/growth factor-mediated mTOR signaling to S6K1. Reports suggest up-regulated expression of LST8 due to insulin, indicating a possible role in intracellular events related to insulin-receptor activation, including insulin-stimulated glucose transport and glycogen synthesis. Northern Blot analysis detected ubiquitous expression of LST8, with highest expression in brain and testis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.