Thermo Fisher Scientific Fluorescein-PGC1a Coactivator Peptide, 100 μM, 100 μL

Fluorescein-PGC1a Coactivator Peptide, 100 μM

Thermo Fisher Scientific LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays.
Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How They Work

Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events:

• Dissociation of repressor proteins
• Association of coactivator proteins
• Assembly of RNA polymerase II and other transcription factors for activation of target genes

TR-FRET–based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding.
These assays can determine:

• The affinity of ligand-bound receptors for different coregulator peptides
• Identification of additional agonists or antagonists via displacement or recruitment of specific coregulator peptides

In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GST-tagged RXR beta protein.
An agonist (9-cis Retinoic Acid) binds to the receptor, causing a conformational change that results in recruitment of the Fl-D22 coactivator peptide.
Binding of the fluorescent coregulator peptide to RXR beta increases the TR-FRET emission ratio.

Contents and Storage

LanthaScreen™ fluorescent nuclear receptor coregulator peptides are supplied as 100 μM solutions in peptide-appropriate buffers.
Store peptides at -20°C.

제품 사양

제품 이미지