Thermo Fisher Scientific SDPR Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

ELISA (ELISA)

1:20,000-1:60,000

-

Immunoprecipitation (IP)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SDPR affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of human SDPR. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SDPR,uniProtId:O95810-1,ncbiNodeId:9606,antigenRange:425,antigenLength:425,antigenImageFileName:600-401-J31_SDPR_O95810-1_Rabbit.svg,antigenImageFileNamePDP:600-401-J31_SDPR_O95810-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.02 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 50% glycerol

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-SDPR specific towards Cavin 2 SDR. A BLAST analysis was used to suggest cross-reactivity with Human based on 100% sequence homology. Cross-reactivity with SDPR from other sources has not been determined.

Target Information

The serum deprivation-response protein (SDPR) is a calcium-independent phospholipid-binding protein whose expression is increased in serum-starved cells. SDPR is a substrate for protein kinase C (PKC) phosphorylation and recruits the polymerase I and transcript release factor (PTRF) to caveolae. Removal of this protein causes caveolae loss and its over-expression results in caveolae deformation and membrane tubulation. Both SDPR and PTRF, as well as the other member of the cavin family PRKCDBP were down regulated in breast cancer cell lines and breast tumor tissue, suggesting that expression of the cavin family proteins could be a useful prognostic indicator of breast cancer progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.